NUTRITION OF CELLULAR SLIME MOLDS II. Growth of Polysphondylium pallidum in Axenic Culture
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چکیده
منابع مشابه
Biochemical Changes during Growth and Encystment of the Cellular Slime Mold Polysphondylium Pallidum
The growth of the cellular slime mold, Polysphondylium pallidum, was studied on a semidefined medium in shaken suspension. When the medium contained large quantities of particulate material, growth was more rapid and the cellular size and protein content were smaller than when growth occurred on a medium containing less particulate material. The cellular levels of DNA, RNA, and protein; of lyso...
متن کاملPhagocytosis by the Cellular Slime Mold Polysphondylium Pallidum during Growth and Development
The phagocytic ability of amoebae of the cellular slime mold Polysphondylium pallidum, grown in shaken suspension, was examined. An established quantitative assay of the uptake of polystyrene (PS) beads was shown to be valid for this organism. The kinetics of phagocytosis were determined, and estimates of the concentration of PS beads necessary to achieve half-maximal phagocytic velocity (K(p))...
متن کاملGrowth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملSpatial patterns in the fruiting bodies of the cellular slime mold Polysphondylium pallidum.
During morphogenesis in the slime mold Polysphondylium pallidum cell masses are periodically pinched off from the base of the developing sorogen. These masses round up and differentiate into secondary sorogens, which become radially ordered arrays of secondary fruiting bodies called whorls. Here we describe the morphogenesis of P. pallidum and characterize the spacing of whorls along the centra...
متن کاملUltrastructural changes during formation and germination of microcysts in Polysphondylium pallidum, a cellular slime mould.
Ultrastructural changes during encystation of Polysphondylium palltdum, a cellular slime mould, include an increase in fibnllar material in the cytoplasmic matrix, the formation of cytoplasms microprojections at the cell periphery and the occurrence of tiny vesicles and some larger vacuoles near the cell periphery. The cyst wall appears first as a fluffy, loose network of fibrils. In the mature...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1963
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.85.1.199-206.1963